Comparison of biological characteristics of nucleus pulposus mesenchymal stem cells derived from non-degenerative and degenerative human nucleus pulposus
نویسندگان
چکیده
منابع مشابه
Influence of simvastatin on the biological behavior of nucleus pulposus-derived mesenchymal stem cells
Objective(s): This research is to study the influences of different concentrations of simvastatin on the biological activities of nucleus pulposus-derived mesenchymal stem cells (NPMSC).Materials and Methods: NPMSC were cultured with different concentrations of simvastatin (0, 0.01, 0.1, and 1 μM) and assessed to determine the possible e...
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OBJECTIVE Our previous work showed that epithelial membrane protein 1 (EMP1) is highly expressed in nucleus pulposus of the human degenerative intervertebral disc. The present study was designed to investigate the role of EMP1 in nucleus pulposus cells in intervertebral disc degeneration (IDD). DESIGN Human nucleus pulposus cells derived from degenerative intervertebral discs were cultured. E...
متن کاملBiologic Response of Degenerative Living Human Nucleus Pulposus Cells to Treatment with Cytokines
PURPOSE To investigate the molecular responses of various genes and proteins related to disc degeneration upon treatment with cytokines that affect disc-cell proliferation and phenotype in living human intervertebral discs (IVDs). Responsiveness to these cytokines according to the degree of disc degeneration was also evaluated. MATERIALS AND METHODS The disc specimens were classified into two...
متن کاملMicroRNA-494 promotes apoptosis and extracellular matrix degradation in degenerative human nucleus pulposus cells
PURPOSE This study investigated the expression and function of the microRNA-494 in intervertebral disc degeneration (IDD). RESULTS MicroRNA-494 expression was upregulated during IDD progression; its overexpression increased the expression of ECM catabolic factors such as matrix metalloproteinase and A disintegrin and metalloproteinase with thrombospondin motif in NP cells while decreasing tha...
متن کاملIL-1β induces apoptosis and autophagy via mitochondria pathway in human degenerative nucleus pulposus cells
IL-1β has been reported highly expressed in degenerative intervertebral disc, and our previous study indicated IL-1β facilitates apoptosis of human degenerative nucleus pulposus (NP) cell. However, the underlying molecular mechanism remains unclear. We here demonstrate that IL-1β played a significantly pro-apoptotic effect under serum deprivation. IL-1β decreased Bcl-2/Bax ratio and enhanced cy...
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ژورنال
عنوان ژورنال: Experimental and Therapeutic Medicine
سال: 2017
ISSN: 1792-0981,1792-1015
DOI: 10.3892/etm.2017.4398